Bioprospecção de fungos basidiomicetos produtores de enzimas oxidativas coletados no município de Boca do Acre-AM

Abstract

Bioprospecting is a way of systematically exploiting nature in search of genetic materials for commercial purposes, and has become a major focus of biotechnology. The term bioprospecting can be understood as the systematic search for organisms, genes, enzymes, compounds, processes and parts from living things that have economic potential and eventually lead to product development. Therefore, it is necessary that we perform a screening of fungi belonging to various groups in order to select for further and deeper studies, those with ligninolytic potential. It is noteworthy that the determination of enzymes a work that consumes time and chemical material not always of low cost, which makes any research in this sense more expensive. In this context, we present the present project where we intend to use the methodology proposed by Bavendamm to perform a preliminary evaluation of Amazon fungi with biotechnological potential. The collection wasperformed in the city of Boca do Acre in the summer period, the strains were labeled and identified in the laboratory, and later bioprospection with tannic acid was performed. Of the twelve Amazonian strains evaluated, five presented halos indicative for phenol oxidases enzymes. However, the greatest activity was evidenced for the species Trametes cubensis, with a mean of halos in 72 hours of activities of 14 and 16 mm, respectively. Despite the favorable result, it is essential to optimize these processes, such as different carbon sources, pH range and also to determine the enzymes of the phenol oxidase class, such as lacase.

Bioprospecting is a way of systematically exploiting nature in search of genetic materials for commercial purposes, and has become a major focus of biotechnology. The term bioprospecting can be understood as the systematic search for organisms, genes, enzymes, compounds, processes and parts from living things that have economic potential and eventually lead to product development. Therefore, it is necessary that we perform a screening of fungi belonging to various groups in order to select for further and deeper studies, those with ligninolytic potential. It is noteworthy that the determination of enzymes a work that consumes time and chemical material not always of low cost, which makes any research in this sense more expensive. In this context, we present the present project where we intend to use the methodology proposed by Bavendamm to perform a preliminary evaluation of Amazon fungi with biotechnological potential. The collection wasperformed in the city of Boca do Acre in the summer period, the strains were labeled and identified in the laboratory, and later bioprospection with tannic acid was performed. Of the twelve Amazonian strains evaluated, five presented halos indicative for phenol oxidases enzymes. However, the greatest activity was evidenced for the species Trametes cubensis, with a mean of halos in 72 hours of activities of 14 and 16 mm, respectively. Despite the favorable result, it is essential to optimize these processes, such as different carbon sources, pH range and also to determine the enzymes of the phenol oxidase class, such as lacase.