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dc.contributor.authorAlmeida, Alex Santos de-
dc.date.available2024-06-06-
dc.date.available2024-06-07T15:43:29Z-
dc.date.issued2020-12-15-
dc.identifier.urihttp://repositorioinstitucional.uea.edu.br//handle/riuea/5708-
dc.description.abstractBacteriophages are viruses that only infect bacteria, do not interact with human cells and are the most abundant microorganisms on the planet. The use of phages has been considered as an alternative measure for the treatment of bacterial infections, mainly against multiresistant antibiotic strains. Salmonella and Shigella are enteropathogens commonly spread by water and food contaminated with fecal material; have a very large intestinal epithelial invasion power, being the most common causes of bacillary dysentery. The present work aimed to isolate and characterize lytic bacteriophages against Salmonella and Shigella strains. The bacteriophages were isolated from water sources, contaminated with domestic effluents, in the city of Manaus- Am. Two serovars of Salmonella enterica subspecies enterica and shigella sonnei were used as hosts for the isolation of species-specific phages; however, their respective infection capabilities have also been analyzed for other bacteria. The isolated phages were analyzed for the nature of their genetic material, infection cycle, morphology and stability. Altogether five lytic bacteriophages were isolated, from Salmonella Typhi, Salmonella Enteritidis and Shigella sonnei, all bacteriophages were able to infect at least ten other bacterial strains; the curves showed the relationships between different ranges of multiplicity of infection (MOI), and determined that the isolated viruses have a relatively low latency period, with releases of high amounts of new viral particles in the "busrt size", the transmission electron microscopy revealed that four of the phages belong to the family Siphoviridae, while a single phage has characteristics of Myoviridae; in the analysis of the genetic material it is not possible to differentiate the phages isolated for S. sonnei by RFLP, however all the other phages had different digestion profiles with the restriction enzymes used. The new phage characterized, in the present work, have promising characteristics to infect and generate lysis not only in host bacteria, but in other species of enterobacteria.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade do Estado do Amazonaspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBacteriófagospt_BR
dc.subjectSalmonella Typhipt_BR
dc.subjectShiguella sonneipt_BR
dc.subjectEnterobacteriaceapt_BR
dc.subjectTerapia Fagicapt_BR
dc.subjectBacteriophagespt_BR
dc.titleIsolamento e caracterização de bacteriófagos líticos para duas espécies de enterobatérias: salmonella esterica subespécie enterica e shigella sonneipt_BR
dc.title.alternativeIsolation and characterization of lytic bacteriophages for two species of enterobacteria: salmonella esterica subspecies enterica and shigella sonneipt_BR
dc.typeDissertaçãopt_BR
dc.date.accessioned2024-06-07T15:43:29Z-
dc.contributor.advisor-co1Oliveira, Hugo Valério Corrêa de-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/6595179051283009pt_BR
dc.contributor.advisor1Astolfi Filho, Spartaco-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/2699190136695057pt_BR
dc.contributor.referee1Oliveira, Hugo Valério Corrêa de-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/6595179051283009pt_BR
dc.contributor.referee2Procópio, Rudi Emerson de Lima-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/2478199435796976pt_BR
dc.contributor.referee3Carmo, Edson Junior do-
dc.contributor.referee3Latteshttp://lattes.cnpq.br/5780309549588357pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/5257152448428326pt_BR
dc.description.resumoRESUMO Os bacteriófagos são vírus que infectam exclusivamente bactérias, não interagem com células humanas e são os microrganismos mais abundantes no planeta. O uso de fagos vem sendo considerado como uma medida alternativa para o tratamento de infecções bacterianas, principalmente contra cepas multirresistentes a antibióticos. Salmonella e Shigella são enteropatógenos comumente disseminados por água e alimentos contaminados com material fecal, possuem um poder de invasão do epitélio intestinal muito grande, sendo as causas mais comuns de disenteria bacilar. O presente trabalho teve como objetivo isolar e caracterizar bacteriófagos líticos contra linhagens de Salmonella e Shigella. Os bacteriófagos foram isolados a partir de fontes hídricas, contaminadas com efluentes domésticos, na cidade de Manaus-Am. Dois sorovares de Salmonella enterica subespécie enterica e shigella sonnei foram utilizadas como hospedeiras para o isolamento de fagos espécie-específicos, contudo, suas respectivas capacidades de infecções também foram analisadas para outras bactérias. Os fagos isolados foram analisados quanto à natureza de seu material genético, ciclo de infecção, morfologia e estabilidade. Ao todo foram isolados cinco bacteriófagos líticos, a partir de Salmonella Typhi, Salmonella Enteritidis e Shigella sonnei, todos os bacteriófagos foram capazes de infectar pelo menos outras nove linhagens bacterianas; as curvas apontaram as relações entre diferentes faixas de multiplicidade de infecção (MOI), e aferiram que os vírus isolados possuem um período de latência relativamente baixo, com liberações de altas quantidades de novas partículas virais no “busrt size”, a microscopia eletrônica de transmissão revelou que quatro dos fagos pertencem a família Siphoviridae, enquanto um único fago possui características de Myoviridae; na análise do material genético não possível diferir os fagos isolados para S. sonnei por RFLP, porém todos os outros fagos possuíam perfis de digestão distintos com as enzimas de restrição usadas. Os novos fagos caracterizados, no presente trabalho, possuem características promissoras para infectar e gerar lise não somente nas bactérias hospedeiras, como em outras espécies de enterobactériaspt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPPGMBT - PROGRAMA DE PÓS-GRADUAÇÃO EM BIOTECNOLOGIA E RECURSOS NATURAIS DA AMAZÔNIApt_BR
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